Veidlapa Nr. M-3 (8)
Study Course Description
DNA in Vitro
Course Code
BUMK_004
Branch of Science
Biological sciences; Environmental biotechnology; Genetics; Medical biotechnology
ECTS
3.00
Target Audience
Dentistry
LQF
Level 7
Study Type And Form
Full-Time
Study Course Implementer
Course Supervisor
Agnese Zariņa
Structure Unit Manager
Juta Kroiča
Structural Unit
Department of Biology and Microbiology
Contacts
Riga, 16 Dzirciema Street, bmk@rsu.lv, +371 67061584
About Study Course
Objective
To provide knowledge acquisition about DNA analysis methods used in clinical practice.
Preliminary Knowledge
Knowledge in biology and chemistry acquired within the framework of general secondary education and basic knowledge in molecular biology.
Learning Outcomes
Knowledge
1.Upon successful completion of the course the students will be able to interpret diagnosis of genetic disease by molecular methods.
Skills
1.Upon successful completion of the course students will be able to extract DNA from living cells, analyse mutation by gel electrophoresis, create a plan for the screening of genetic disease.
Competences
1.Students will be able to assemble theoretical knowledge and practical skills and to justify necessity of its usage in solution of clinical problems.
Assessment
Individual work
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Title
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% from total grade
|
Grade
|
|---|---|---|
|
1.
Individual work |
-
|
-
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|
To prepare literature review about latest achievements in molecular genetics relevant in dentistry. Individual work has 50% impact on the final evaluation.
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Examination
|
Title
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% from total grade
|
Grade
|
|---|---|---|
|
1.
Examination |
-
|
-
|
|
Attendance of practical classes will be registered, implementation of laboratory work protocols will be controlled, evaluation of practical skills will be carried out. At the end of the course it is possible to obtain a cumulative assessment, which consists of activities in practical classes and independent work.
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Study Course Theme Plan
FULL-TIME
Part 1
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
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On site
|
Auditorium
|
2
|
Topics
|
Horizontal and vertical electrophoresis basic principles. Analysis of PCR products in agarose gel. Restriction fragment length polymorphism analysis (RFLP).
|
-
Class/Seminar
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Modality
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Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Polyacrylamide gel electrophoresis (PAAGE).
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-
Class/Seminar
|
Modality
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Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
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Topics
|
Analysis of the obtained results. Reports for the results of diagnostic genetic testing preparation.
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-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
The secrets of DNA molecule. The structure of Human genome. Mutations. DNA polymorphism. The role of genetics in clinical dentistry.
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-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
DNA extraction from mouth bacteria. Sampling methods and creating sample groups. Verification of reagents.
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-
Class/Seminar
|
Modality
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Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Student portfolio presentations.
|
-
Lecture
|
Modality
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Location
|
Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
2
|
Topics
|
Genetic aspect of periodontitis.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Validation of the obtained results by using sequencing method as an example.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
2
|
Topics
|
DNA restriction and ligation.
|
-
Class/Seminar
|
Modality
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Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Polymerase chain reaction (PCR).
|
-
Class/Seminar
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Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
2
|
Topics
|
Programmes for DNA analysis.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Analysis of obtained results. Significance of the results in molecular diagnosis of periodontitis.
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-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Extraction solution preparation required for DNA. DNA extraction, extracted DNA quality assessment.
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-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Introduction to laboratory equipment and work in scientific laboratories. Individual experimental plan creation. Pipetting.
|
-
Class/Seminar
|
Modality
|
Location
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Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
2
|
Topics
|
DNA sequencing.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
2
|
Topics
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Topicalities in DNA analysis.
|
Total ECTS (Creditpoints):
3.00
Contact hours:
32 Academic Hours
Final Examination:
Exam (Written)
Bibliography
Required Reading
1.
Russell P.J. Genetics: A Molecular Approach. 3rd edition, Pearson, 2013.
2.
WS Klug, MR Cummings, ChA Spencer, MA Palladino, D Killian. Concepts of Genetics. Pearson, 12th ed., 2018.
Additional Reading
1.
Strachan T., Read A. Human Molecular Genetics. - GS, 2011
Other Information Sources
1.
Internets. NCBI (DNA &RNA, Genetics&Medicine, Genes &Expression, Genomes&Maps). Papildus saites tiks norādītas studiju kursa apguves laikā.