Horizontal Gene Transfer

Title	% from total grade	Grade
1. Individual work	-	-
Each student prepares and presents one 10-minute presentation about topic of their choice (topic must be related to the at least one of the themes learned in this course). Presentations are presented at the last classes of this course. In order to evaluate the quality of the study course as a whole, the student must fill out the study course evaluation questionnaire on the Student Portal.

Title	% from total grade	Grade
1. Examination	-	-
Practical work (presentation) assessment criteria: visual material in presentation, speech and flow of story of presenter, answers to the questions and discussion about the topic of presentation. In addition, the assessment is performed by other students. Only teacher's assessment is taken into account for cumulative grade of the course. E-tests and exam is graded based on point system uploaded in e-studies.

Introductory lecture: types of gene transfer, horizontal gene transfer (HGT) in prokaryotes and eukaryotes, mobile genetic elements, HGT and evolution, introduction in gene engineering. Description Literature: Vogan and Higgs, 2011, The advantages and disadvantages of horizontal gene transfer and emergence if the first species. Biology Direct (https://biologydirect.biomedcentral.com/articles/10.1186/1745-6150-6-1) Crisp et al., 2015, Expression of multiple horizontally acquired genes is a hallmark of both vertebrate and invertebrate genomes. Genome Biology (https://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0607-3) Yahara et al., 2012, Genome-Wide Survey of Mutual Homologous Recombination in a Highly Sexual Bacterial Species. Genome Biol.Evol., 4, 628-640 (https://academic.oup.com/gbe/article/4/5/628/535531) Alberts, Mol Biol of the Cell, 5th Edit, 2008 http://preuniversity.grkraj.org/html/10_MOLECULAR_BIOLOGY.htm Ravenhall M, Škunca N, Lassalle F, Dessimoz C (2015) Inferring Horizontal Gene Transfer. PLoS Comput Biol.

Recombinant DNA technologies and their use in biomedicine (principles of construct design). Manipulation of nucleic acids, cloning genes for synthetic biology (vectors, expression systems). Description Literature: https://geneticeducation.co.in/different-types-of-dna-extraction-methods/ Yin et al., 2007 Journal of Biotechnology 127: 335–347 https://www.youtube.com/watch?v=zm-3kovWpNQ

Overview of Gene Therapy.

Overview of immunotherapy (CAR-T cells).

Designing the gene constructs (laptops are needed). How to prepare for 10-min talk (practical tips for presentations)?

Seminar "Meet the author", scientific paper analysis, online seminar with author (in English). Description Annotation: Preparation needed for this class - read the scientific paper posted in e-studies.

DNA isolation and discussion of other analytical methods.

Artificial Intelligence tools for research.

Cell therapy and tissue engineering.

Bioethical aspects of genetic manipulations of human genetic material (guest lecture). Description Literature: https://ghr.nlm.nih.gov/primer/therapy/genetherapy https://pink.pharmaintelligence.informa.com/PS143536/EU-Gene-Therapy-Submissions-Approvals-Expected-To-Pick-Up-In-2021 https://www.fda.gov/vaccines-blood-biologics/cellular-gene-therapy-products/approved-cellular-and-gene-therapy-products Hacein-Bey-Abina et al., 2002, NEJM https://www.cancer.gov/about-cancer/treatment/research/car-t-cells Simonato et al., 2013, Nature Reviews Neurology Sharpe and Mount, 2015, Disease Models & Mechanisms Johnson and June, 2017, Cell Research The Journal of Gene Medicine http://www.genetherapynet.com/regulatory-affairs.html

Biological aspects of genetic manipulations with human material.

Gene engineering: transgenic animals and plants

Practical work – bacterial transformation with plasmid DNA (in DNA class) or seminar "Meet the embryologist" online.

Students' presentation, Q and A, discussion.

Students' presentation, Q and A, discussion.

Students' presentation, Q and A, discussion.