Veidlapa Nr. M-3 (8)

Study Course Description

Bacteriology and Diagnostics of Tuberculosis

Main Study Course Information

Course Code

RLA_025

Branch of Science

Clinical medicine; Microbiology and Virology

ECTS

30.00

Target Audience

Medicine

LQF

Level 8

Study Course Implementer

Course Supervisor

Jeļena Storoženko

Residency Speciality

Speciality

Laboratory doctor

Supervisor of Medical Speciality

Jeļena Storoženko

Contacts

About Study Course

Objective

To prepare laboratory doctors capable of performing a wide spectrum of examinations for diagnostics of bacterial infections, incl. tuberculosis, using modern technologies and equipment. To use examination algorithms based on international guidelines and recommendations, to interpret the obtained results. To master internal and external quality control methods for diagnostics of bacterial infectious diseases.

Learning Outcomes

Knowledge

1.- to evaluate advantages of different groups of diagnostic methods; - to describe principles of diagnostic methods used in bacteriology; - to characterise properties of pathogenic cocci and pathogenic groups of intestinal microorganisms; - to evaluate the use of immunoenzymatic and molecular biological methods in bacteriology; - to describe rules of sampling biological material for diagnostics of bacterial infections.

Skills

1.- to prepare samples for bacteriological examination; - to perform inoculation of the material to be examined, sampling of colonies for pure culture; - to perform microscopy of stained preparations; - to determine sensitivity of microorganisms to antibiotics and to interpret the results - to use conventional and unified method algorithm for identification of microorganisms.

Competences

1.To perform diagnostic examinations of human biological material for diagnostics of bacterial infections. Based on exercise results to perform diagnostics, differential diagnostics of diseases, to interpret results and to recommend further examination activity to clinicians.

Assessment

Individual work

Title	% from total grade	Grade
1. Individual work	-	-
Preparation of a presentation on anaerobic microorganisms, identification of agents causing diarrhoea. In-depth research: problems of antibiotic resistance of microorganisms.

Examination

Title	% from total grade	Grade
1. Examination	-	-
Assessment of knowledge – written answers to questions at the end of the study course.

Study Course Theme Plan

FULL-TIME

Part 1

Seminar

Modality	Location
On site	Auditorium

Topics

Bacteriological testing methods: microscopy, culturing, cytological, serological, allergological

Seminar

Modality	Location
On site	Auditorium

Topics

Morphology and physiology of microorganisms. Methods of microorganism identification.

Seminar

Modality	Location
On site	Auditorium

Topics

Problems of antimicrobial resistance. Determination of antibiotic susceptibility

Seminar

Modality	Location
On site	Auditorium

Topics

Diarrhea causing agents, their proportion in the clinic and their identification methods

Seminar

Modality	Location
On site	Auditorium

Topics

Anaerobic microorganisms: isolation, identification, diseases caused by them. Microanaerophilic microorganisms

Seminar

Modality	Location
On site	Auditorium

Topics

Pathogenic cocci

Seminar

Modality	Location
On site	Auditorium

Topics

AIDS - related bacterial and fungal infections.

Seminar

Modality	Location
On site	Auditorium

Topics

Diagnosis of dangerous bacterial infections

Seminar

Modality	Location
On site	Auditorium

Topics

Diagnosis of bacterial respiratory infections and invasive bacterial infections

Seminar

Modality	Location
On site	Auditorium

Topics

Laboratory diagnostics of rare and quarantine infectious agents

Seminar

Modality	Location
On site	Auditorium

Topics

Automated examination methods in microbiology

Seminar

Modality	Location
On site	Auditorium

Topics

Laboratory diagnostics of tuberculosis

Seminar

Modality	Location
On site	Auditorium

Topics

Identification and drug susceptibility of tuberculosis mycobacteria

Seminar

Modality	Location
On site	Auditorium

Topics

International guidelines for the diagnosis of tuberculosis

Seminar

Modality	Location
On site	Auditorium

Topics

M.tuberculosis genomes. Genome organization. Genomics. Use of characteristic genetic elements in the diagnosis of tuberculosis

Total ECTS (Creditpoints):

30.00

Number of Residency Seminars:

Length (weeks):

Final Examination:

Residency exam (Theory and practice)

Bibliography

Required Reading

Henry's Clinical Diagnosis and Management by Laboratory Methods. 23rd Edition, 2017

Preanalytical Phase Errors: Experience of a Central Laboratory Monitoring. Editor: Alexander Muacevic and John R. Adler; e7335. Published online 2020 Mar 20. doi: 10.7759/cureus.7335

Manual of Clinical Microbiology. 2 Volume Set, 12th Edition. Ed: Karen C. Carroll, Michael A. Pfaller, Marie Louise Landry. February 2019, ASM Press

Current and Emerging Technologies for the Diagnosis of Microbial Infections. Volume 42. Ed: A. Sails, Yi Wei Tang. Published by Academic Press, 2015

Laboratory Diagnosis of Tuberculosis. Latest Diagnostic Tools. Gunes Senol, 2013

Sarah Jane Pitt. Clinical Microbiology for Diagnostic Laboratory Scientists. 2018

Additional Reading

Clinical Microbiology Procedures Handbook. Published by American Society for Microbiology. United States, 2016

Global tuberculosis report. 2021

Human Microbiota and Microbiome. Edited by Julian K. Marchesi, CABI, 2014

Other Information Sources

Practical Laboratory Medicine, Journal. Co Editors-in-Chief: William Clarke, Mike J. Hallworth

http://www.gfmer.ch/Medical_journals/Infectious_diseases_microbiology_tropical_medicine.htmhttp://www.ncbi.nlm.nih.gov/pmc/journals/85/

Journal of Clinical Microbiology

http://www.intechopen.com/books/understanding-tuberculosis-global-experiences-and-innovative-approaches-to-the-diagnosis/clinical-laboratory-diagnostics-for-mycobacterium-tuberculosis