Veidlapa Nr. M-3 (8)
Study Course Description
Experimental Research in Medical Chemistry
Course Code
CFUBK_025
Branch of Science
Chemical sciences
ECTS
3.00
Target Audience
Biology; Civil and Military Defense; Life Science; Medicine
LQF
Level 7
Study Type And Form
Full-Time
Study Course Implementer
Course Supervisor
Āris Kaksis
Structure Unit Manager
Pēteris Tretjakovs
Structural Unit
Department of Human Physiology and Biochemistry
Contacts
Riga, 16 Dzirciema Street, cfbk@rsu.lv, +371 67061550
About Study Course
Objective
Study and experimentally investigate: Structure, composition, biochemical and physiological functions of proteins, lipids, carbohydrates and nucleic acids in the human body.
Preliminary Knowledge
Languages, computer skills and skills to study the Internet on the web (written and oral in the scope of the high school program); natural sciences and mathematics: chemistry, mathematics (algebra and geometry), biology, physics.
Learning Outcomes
Knowledge
1.Knowledge how to research molecules using IT technologies for skills: • to formulate and to explain Medical Chemistry and Static Biochemistry concepts; • to describe quantitative and qualitative concepts of Medical Chemistry processes; • to estimate concentration of oxygen, glucose, CO2, blood pH and osmomolar and concentration gradient on membrane; • to explain potential principles of ion channel made membrane; • to contrast the effects of natural substances and harmful substances used in a healthy diet on health.
Skills
1.Student will be able to analyse metabolism equilibria and homeostasis stepwise. Able to estimate metabolism and exchange in equivalent amounts, based on proteins-enzymes mechanisms. Enable skills to analyze enzymes building and structure influence, that appreciate interaction with environment and integrate metabolism for maintenance of life, what reflect students results on work papers as conclusions, tests and solutions.
Competences
1.Students know how to analyse biologic compound exchange and states of homeostasis. They will know how to estimate compound exchange and conversion in equivalent amounts, based on mass conservation and energy conservation rules. They will be able to analyse atomic properties of chemical elements integration and conversion into molecular buildings and aggregate properties, to estimate interaction with environment and integrated compound exchange for maintenance of living functions.
Assessment
Individual work
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Title
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% from total grade
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Grade
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1.
Individual work |
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-
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Individual and pair work in practical preparation of protocol and notes. Study of literature given Text books, in the list of readings, including scientific publications.
In order to evaluate the quality of the study course as a whole, the student must fill out the study course evaluation questionnaire on the Student Portal.
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Examination
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Title
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% from total grade
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Grade
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1.
Examination |
-
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-
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work paper cumulative course assessment form for final evaluation of semester results
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2.
Examination |
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10 points
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Checking the quality of written solutions for the tasks, questions and problem solutions set in the practical classes in the study protocols of independent experiments. Written tests to test skills and abilities. Colloquia – examination of the acquired theoretical and practical knowledge and skills, in which the understanding of the study material is confirmed. Written final exam. |
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Study Course Theme Plan
FULL-TIME
Part 1
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Class/Seminar
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Modality
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Location
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Contact hours
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On site
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Laboratory
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3
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Topics
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Install applications on your computer.
Computer preparation for molecules experimental research. Installation.
Medical Bio Chemistry data base.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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3
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Topics
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Aquaporins cell membranes crosing H2O, O2, NO transport proteins of enzyme class E7. The osmosis against concentration gradient in human body for irreversible driven homeostasis.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Auditorium
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3
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Topics
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Carbonic Anhydrase CA ENZYME class E3. Primary attractor pH=7.36 is perfect homeostasis functional activator; irreversible CO2 assimilation in activate bicarbonate form and massive limestone, dolomite, chalk stone and marble mountains creator on planet Earth.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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3
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Topics
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O2, H+, HCO3- shuttles Hemoglobin, Myoglobin, Carbonic Anhydrase CA molecules. Triplet state •:O≡:::≡O:• oxygen. Oxygen O2, CO2aqua/HCO3‑ +H+ shuttle mechanism of transport ENZYMES E7+E3 classes complex in human organism.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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2
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Topics
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Heme Tyr357 coordinated iron(III) ion Fe3+ collision with deprotonate -OOH peroxide anion CATALASE activation energy is Ea=29 kJ/mol, geometric factor A=0,13. Indispensable Brownian molecular engine irreversible homeostasis instrument for evolution, life and survival.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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2
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Topics
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Cyclo oxigenase eicosanoids synthase with singlet •::O-:-O::• oxygen on active site of peroxidase. Eicosatetraenoic acid. Source of: prostaglandins PGs, prostacyclins PGIs, thromboxanes TXs and leukotrienes. LTs. The COX synthesis inhibitors: aspirin, warfarin, tylenol, paracetamol, ibuprofen.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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2
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Topics
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ENZYME alcohol dehydrogenase ADH. B3 vitamin with hydride ion H‑ tunnelling oxidise alcohol to aldehyde. Hydride ion removing and proton H+ dissociation.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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3
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Topics
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Cell membranes lipids composite structure at homeostasis concentration gradients generation for transport down and for osmosis against concentration gradients. Holesterol 0,9÷1/1 phospholipid ratio in human erytrocite and transport enzymes substance (aquaporins, myoglobin, haemoglobin, COX). Steroid Steroidogenic Acute Regulatory protein-related lipid Transfer START and 13 domains STARD1-13 of proteins.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Auditorium
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3
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Topics
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Human serum albumin HSA HOMEOSTASIS concentration in blood. HSA upload in water insoluble 7 fatty acids with total concentration 0,6 mM*7=4,2 mM. Transport in water insoluble Hem, bilirubin, testosterone and insoluble medicine aspirin, warfarin, ibuprofen, indometacin, ect.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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3
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Topics
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Androgen nuclear receptors. Mineral corticoid receptors. DNA binding Zn finger motifs for treatment
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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2
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Topics
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Genom HOMEOSTASIS instruments DNA methyl transferases.
Methylation Protein DNMT3, DNMT1, HhaI experimental research task.
Zn2+ ions DNA Medical Chemistry zinc finger motifs on DNA phosphate strands.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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3
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Topics
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http://aris.gusc.lv/ChemFiles/Aquaporins/AquaPorin1.htm. Aquaporins cell membranes crosing transport proteins for H2O, O2, NO molecules osmosis against osmolar concentration gradient in human body.
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Total ECTS (Creditpoints):
3.00
Contact hours:
32 Academic Hours
Final Examination:
Exam (Written)
Bibliography
Required Reading
1.
David L. Nelsone, Michael M. Cox. Lehninger Principles of Biochemistry. 2017. W.H. Freeman and Company, New York.
2.
Literature: http://aris.gusc.lv/2024MFLatBkurss.pdf; 2023. gada 36 publications
Additional Reading
1.
Biochemie des Menschen. Horn F. 8th ed., 2020.
Other Information Sources
1.
36 Publikācijas izvēlētajos Medicīniskās ķīmijas tematos 1. un 2. lapaspusē /36 Publications in selected Medicinal Chemistry topics on pages 1 and 2