Veidlapa Nr. M-3 (8)
Study Course Description
Cell and Tissue Microstructure
Course Code
MK_032
Branch of Science
Clinical medicine; Histology and Cytology
ECTS
3.00
Target Audience
Medical Technologies
LQF
Level 6
Study Type And Form
Full-Time
Study Course Implementer
Course Supervisor
Valērija Groma
Structure Unit Manager
Māra Pilmane
Structural Unit
Department of Morphology
Contacts
Riga, 9 Kronvalda boulevard, aaiak@rsu.lv, +371 67061551
About Study Course
Objective
This course is designed to deepen our knowledge of fundamental cellular and molecular biology problems, to develop practical skills and competences in cell structure microscopical and ultrastructural analysis in the medical engineering sector. The course focuses on the essential principles of cell structure and activities accompanied by a description of commonly used techniques and equipment. This course provides the possibility to combine a theoretical knowledge and practical demonstrations, and increase understanding of the cellular base of physiological and pathological processes and their significance in the study of medical engineering.
Preliminary Knowledge
Previously acquired knowledge of biology and chemistry.
Learning Outcomes
Knowledge
1.Students will be able to describe basic properties of cells and main cell types, history of discovery of the cell, modern methods used for the study of cells, components of biological membranes and their role in the maintenance of cell function, cellular organelles and main events attributed to the cytoplasmatic organelles, tissue adaption, tissue barriers, biocompatibility; cell and tissue examination methods and novel future possibilities.
Skills
1.Using bright-field optics and examining paraffin-embedded tissue sections, students will be able to distinguish the major cell components, will be introduced to commonly used cytological staining techniques; will be able to evaluate a specialized cell shape and size, and structurally based communications, as well as identify major artifacts appearing due to inadequate sample processing. Students will be able to describe and distinguish main tissue groups and organ systems, describe modern morphology methods and their necessary equipment.
Competences
1.Student will be able to distinguish by the use of a microscope: main tissue groups, structure of the main organs; evaluate quality of a particular slide, interactions and localization of tissue structures within the histological slide; will be able to document cell biology and histology slides and to work with the special literature.
Assessment
Individual work
|
Title
|
% from total grade
|
Grade
|
|---|---|---|
|
1.
Individual work |
-
|
-
|
|
Analysis of the electron and light microscopy micrographs, descriptions of schemes that confirm the understanding of fundamentals of cell biology. Self-studies using literature, making a description of electron micrographs, histological slides, and schemes. Descriptions and contribution to the discussion related to slide analysis.
|
||
Examination
|
Title
|
% from total grade
|
Grade
|
|---|---|---|
|
1.
Examination |
-
|
-
|
|
Attendance of practical classes and lecture, practical work during practical class (20%), analysis of histological slides, cellular structures. Participation in a discussion (30%). Written Exam at the end of this study course (50% of evaluation).
|
||
Study Course Theme Plan
FULL-TIME
Part 1
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
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Other
|
2
|
Topics
|
Diversity of cells. Morphological research methods.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
The cell membrane. Molecular transport through membranes. Cytoplasmic organelles.
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-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Epithelial tissue and connective tissue. Tissue barriers in the human body.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Supporting tissues, the dynamic nature and remodeling of supportive tissue. Biocompatibility.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Muscle tissue. Muscle tissue contraction mechanism. Muscle tissue regeneration.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Nervous tissue, structure and functions. The concept of the cell membrane action potential; nerve impulse. Neurotransmitters.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Blood cell morphology; cardiovascular system (heart, blood vessels), the reconstruction, vascular stents.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
3
|
Topics
|
Diversity of eukaryotic cells; preparation of histological slides; immunocytochemistry, cytochemistry, clinical significance.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
3
|
Topics
|
Method of electron microscopy; semithin and ultrathin sectioning; preparation and analysis.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
3
|
Topics
|
Demonstrations of various epithelia and connective tissue (cornea, skin, intestinal epithelium, loose connective tissue, tendon).
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-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Other
|
3
|
Topics
|
Cartilage.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Other
|
3
|
Topics
|
Various types of bone, bone implants.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
3
|
Topics
|
Nervous tissue, structure and functions; neural structure of the synapse and neurotransmitters; impulse transmission; myelin; regeneration.
|
Total ECTS (Creditpoints):
3.00
Contact hours:
32 Academic Hours
Final Examination:
Exam (Written)
Bibliography
Required Reading
1.
Groma V., Zalcmane V. Šūna: uzbūve, funkcijas, molekulārie pamati. 2012. Rīga, RSU. 284 lpp. Mācību grāmatas autore ir struktūrvienības darbiniece, no latviešu grāmatu klāsta, patlaban vienīgais pieejamais avots./The author of the textbook is an employee of the structural unit, from the range of Latvian books, currently the only available source.
2.
Kierszenbaum Abraham L., Tres Laura L. 2019. Histology and Cell Biology: An Introduction to Pathology. 5th Edition. Elsevier. 824 p. ISBN: 9780323673211, eBook ISBN: 9780323683784
3.
Burns E.R., Cave M.D. 2007. Histology and Cell Biology. 2nd Edition. Mosby. 321 p. ISBN-13: 9780323044257, ISBN-10: 0323044255 (akceptējams izdevums/approved publication)
4.
Paulsen D.F. 2021. Histology and cell biology: Examination and Board Review. 6th Edition. McGraw Hill/ Medical. 453 p. ISBN 978-1-264-26992-1, ISSN 1045-4586
5.
Allen T. 2015. Microscopy: A Very Short Introduction. Oxford University Press. 144 p. ISBN-13: 978-0198701262, ISBN-10: 9780198701262
6.
Eroschenko Victor P. 2013. diFiore's Atlas of Histology: with Functional Correlations (Atlas of Histology (Di Fiore's)). 12th Edition. LWW. 617 p. ISBN-13: 978-1496316769, ISBN-10: 1496316762
7.
Mescher Anthony L. 2021. Junqueira's Basic Histology: Text and Atlas. 16th Edition. McGraw-Hill Education. 480 p. ISBN-10:1260462986, ISBN-13:978-1260462982
8.
Lowe J., Anderson P., Anderson S. 2018. Stevens & Lowe's Human Histology. 5th Edition. Elsevier. 440 p. ISBN: 9780323612807, ISBN: 9780323612791
9.
Ārvalstu studentiem/For international students:
10.
Kierszenbaum Abraham L., Tres Laura L. 2019. Histology and Cell Biology: An Introduction to Pathology. 5th Edition. Elsevier. 824 p. ISBN: 9780323673211, eBook ISBN: 9780323683784
11.
Burns E.R., Cave M.D. 2007. Histology and Cell Biology. 2nd Edition. Mosby. 321 p. ISBN-13: 9780323044257, ISBN-10: 0323044255 (akceptējams izdevums/approved publication)
12.
Lowe J., Anderson P., Anderson S. 2018. Stevens & Lowe's Human Histology. 5th Edition. Elsevier. 440 p. ISBN: 9780323612807, ISBN: 9780323612791
13.
Mescher Anthony L. 2021. Junqueira's Basic Histology: Text and Atlas. 16th Edition. McGraw-Hill Education. 480 p. ISBN-10:1260462986, ISBN-13:978-1260462982
14.
Eroschenko Victor P. 2013. diFiore's Atlas of Histology: with Functional Correlations (Atlas of Histology (Di Fiore's)). 12th Edition. LWW. 617 p. ISBN-13: 978-1496316769, ISBN-10: 1496316762
Additional Reading
1.
Boka S., Pilmane M., Kavak V. 2010. Embryology and anatomy for health sciences. Rīgas Stradiņa universitāte. 404 p. ISBN-10: 9984788504, ISBN-13: 978-9984788500. Mācību grāmatas autore ir struktūrvienības darbiniece, no latviešu grāmatu klāsta, patlaban vienīgais pieejamais avots./The author of the textbook is an employee of the structural unit, from the range of Latvian books, currently the only available source.
2.
Young Barbara, O'Dowd Geraldine, Woodford Phillip. 2013. Wheater's Functional Histology: A Text and Colour Atlas. 6th Edition. Churchill Livingstone. 464 p. ISBN-10: 0702047473, ISBN-13: 978-0702047473
3.
Ovalle William K., Nahirney Patrick C. 2020. Netter's Essential Histology. 3rd Edition. Elsevier. 568 p. ISBN-10:0323694640, ISBN-13:978-0323694643
Other Information Sources
1.
Vrana N. E. 2020. Cell and Material Interface: Advances in Tissue Engineering, Biosensor, Implant, and Imaging Technologies (Devices, Circuits, and Systems). 1st Edition. CRC Press. 294 p. ISBN-13: 978-0367656379, ISBN-10: 036765637X
2.
Freeman J. W. , Banerjee D. 2018. Building Tissues: An Engineer's Guide to Regenerative Medicine (Biomedical Engineering). 1st Edition. CRC Press. 233 p. ISBN-13: 978-1498742801, ISBN-10: 1498742807