Veidlapa Nr. M-3 (8)
Study Course Description
Medical Microbiology
Course Code
BUMK_015
Branch of Science
Clinical medicine; Microbiology and Virology
ECTS
12.00
Target Audience
Medicine
LQF
Level 7
Study Type And Form
Full-Time
Study Course Implementer
Course Supervisor
Juta Kroiča
Structure Unit Manager
Juta Kroiča
Structural Unit
Department of Biology and Microbiology
Contacts
Riga, 16 Dzirciema Street, bmk@rsu.lv, +371 67061584
About Study Course
Objective
To provide knowledge about structure and qualities of bacteria, viruses, fungi and parasites and their role in infectious disease.
To provide knowledge about innate and adaptive immune response.
To inform about bacteriological, virological and serological diagnostic methods. Diagnostics of fungal and parasitic infections.
To provide knowledge about antibiotics, their mechanisms of action and mechanisms of antibacterial resistance.
Preliminary Knowledge
Molecular Biology, Anatomy, Histology, Genetics, Physiology and Biochemistry.
Learning Outcomes
Knowledge
1.On completion of the course the students will be able to describe bacteria, viruses, fungi and parasites, to understand their pathogenic factors as well as their role in the origin of infectious diseases and in the interrelation with host. They will be informed about the main methods of diagnostics, unspecific and specific methods of prevention of infectious diseases.
Skills
1.On completion of the course the students will be able to: • collect, transport and examine patients' material; • identify the pure culture of the causautive agents, evaluate their antibacterial susceptibility; • use bacteriological, serological and molecular methods.
Competences
1.Upon combining the theoretical knowledge and practical skills, the students will be able to understand the significance of viruses, bacteria, fungi and parasites as causative agents of diseases and to relate them to the mechanisms of pathogenecity and interreaction with the host.
Assessment
Individual work
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Title
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% from total grade
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Grade
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|---|---|---|
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1.
Individual work |
-
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-
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|
Within the framework of the course, students work individually and in groups. The preparation of presentations in accordance with the topics of the course.
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Examination
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Title
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% from total grade
|
Grade
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|---|---|---|
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1.
Examination |
-
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-
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• Regular attendance of practical classes and active participation in them.
• Passed semester colloquia. A colloquium includes the assessment of theoretical knowledge and practical skills.
• After 3rd semester – a test (written).
• At the end of the course – a written examination consisting essay-type questions and questions regarding practical classes.
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Study Course Theme Plan
FULL-TIME
Part 1
-
Lecture
|
Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Auditorium
|
2
|
Topics
|
Brief history of microbiology. Taxonomy of microorganisms. The structure of bacterial cell.
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-
Lecture
|
Modality
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Location
|
Contact hours
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|---|---|---|
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On site
|
Auditorium
|
2
|
Topics
|
Normal human microbiota and microbiota of an environment. Control of microbial growth. Infection.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Bacterial metabolism. Microbial growth. The atypical bacteria, fungi, viruses. Bacterial pathogenicity.
|
-
Lecture
|
Modality
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Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Antibiotics. Microorganisms resistance against antibiotics. Mechanisms of resistance.
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-
Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Safety in microbiology laboratory. Microscopy. Microbiological smears. Simple methods of staining.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Complex methods of staining. The morphology of bacterial cell - endospores, flagella, inclusion bodies.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Specialized room
|
3
|
Topics
|
Cultivation of bacteria. Media. The atypical bacteria, fungi, viruses.
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-
Class/Seminar
|
Modality
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Location
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Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Pure cultures. Cultivation of anaerobic microorganisms. Identification of pure cultures. Cultural, morphological, biochemical properties of bacteria. Sampling of normal microbiota of human body.
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-
Class/Seminar
|
Modality
|
Location
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Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Normal human and environment microbiota. Principles of examination and evaluation of microbiota.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Examination of antibacterial susceptibility. Disinfection methods. Sterilisation methods.
|
-
Lecture
|
Modality
|
Location
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Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Immunity, classification of it. Non-specific resistance of organism. General and cellular factors – phagocytosis, inflammation, TLR, mechanisms, outcomes.
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-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
|
Auditorium
|
2
|
Topics
|
Humoral factors of unspecific resistance. Complement system, activation factors, outcomes.
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-
Lecture
|
Modality
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Location
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Contact hours
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|---|---|---|
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On site
|
Auditorium
|
2
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Topics
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Antigens, their structure. MHC-I and MHC-II class antigens, their properties. Antibodies, their structure, properties. Ig classes.
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-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
T Lymphocytes, hystiogenesis, receptors, functions. NK cells.
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-
Lecture
|
Modality
|
Location
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Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
B ly, Macrophages, APC. Primary and secondary humoral immune response.
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-
Lecture
|
Modality
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Location
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Contact hours
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|---|---|---|
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On site
|
Auditorium
|
2
|
Topics
|
Cellular immune response. Antiviral immune response.
Antitoxic, antifungal, anticapsular immune response.
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-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Hypersensitivity, characteristics, types of reactions and mechanisms.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Interaction of micro and macroorganisms. Bacterial pathogenicity factors and role of human protective factors in origin of infection.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Serological reactions. Diagnosticums. Immune sera. Agglutination reactions.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Precipitation test. Lytic reactions.
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-
Class/Seminar
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Modality
|
Location
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Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
The use of labeled antibodies and antigens in serological reactions (ELISA, IFA). PCR.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Antiviral, antitoxic, antifungal, anticapsular immune response.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Immune response. Immunization mechanisms, mechanisms of immune prophylaxis.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Immune pathology, hypersensitivity.
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-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Microbiological diagnostic algorhytm of microorganisms.
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Total ECTS (Creditpoints):
6.00
Contact hours:
64 Academic Hours
Final Examination:
Exam (Written)
Part 2
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
2
|
Topics
|
Enterobacteriaceae, E.coli, Salmonella spp.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Shigella. Yersinia. Campylobacter. Helicobacter. Cholera.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Anaerobic bacteria. Corynebacterium diphtheria. Hospital acquired infection. Clinical bacteriology.
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-
Lecture
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Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Pathogenic coccus.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Pathogenic Spirochettae. Mycoplasma. Chlamydia.
|
-
Lecture
|
Modality
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Location
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Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
The causative agents of Tuberculosis and Actinomycosis.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Pathogenic fungi.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Microbiological diagnosis of E.coli, Salmonella spp infections.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Microbiological diagnosis of the diseases caused by Yersinia, Shigella, Campylobacter, Helicobacter and Vibrio cholerae.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Microbiological diagnosis of diseases caused by Staphylococcus spp. and Streptococcus spp., gonococcus and meningococcus.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Microbiological diagnosis of diphtheria and infections caused by genus Clostridium.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Microbiological diagnosis of Tuberculosis, Mycobacteria spp., Actinomycosis.
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-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Syphilis, Borreliosis and Leptospirosis. Diagnostic tests of diseases caused by Chlamydia, Mycoplasma.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
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|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Clinical bacteriology. Examination of pus, blood, sputum and urine, cerebro-sprinal fluid. Diagnostic tests of diseases caused by Fungi (Dermatophytes, Candida, Cryptococcus).
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Orthomyxoviridae, paramyxoviridae. Herpesviruses. Adenoviruses.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Enteroviruses. Hepatitis A,B,C,D,E.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Neurotropic viruses. Slow viral infections. Oncovirology. HIV. AIDS.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Causative viral agents of respiratory infections. Principles of viral diagnostics. Diagnostics of respiratory viruses.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Diagnostic tests of the Enteroviruses and diseases caused by Hepatitis Viruses.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Virological diagnostics of neurotropic viral infections (Tick-born encephalitis and rhabies). Diagnostic principles of HIV, AIDS.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Introduction to medical parasitology. Classification of parasites. Helminths– trematodes (Trematoda) and cestodes (Cestoda). Parasitic protists (Protozoa), their classification. Their caused diseases in human.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Helminths – nematodes (Nematoda). Parasitic arthropods (Arthropoda), their significance as vectors. Vector borne diseases.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Protozoa - Trichomonas vaginalis, Giardia duodenalis, Entamoeba spp., Acanthamoeba spp., Balantidium coli, Toxoplasma gondii, Plasmodium spp. Helminths – trematodes, their characteristics: Fasciola hepatica, Schistosoma spp. Cestodes, their characteristics: Taenia solium, T. saginata, Diphyllobothrium latum, Hymenolepis nana, Echinococcus granulosus, Echinococcus multilocularis.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Protozoa - Trichomonas vaginalis, Giardia duodenalis, Entamoeba spp., Acanthamoeba spp., Balantidium coli, Toxoplasma gondii, Plasmodium spp. Helminths – trematodes, their characteristics: Fasciola hepatica, Schistosoma spp. Cestodes, their characteristics: Taenia solium, T. saginata, Diphyllobothrium latum, Hymenolepis nana, Echinococcus granulosus, Echinococcus multilocularis.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Helminths – nematodes, their characteristics: Enterobius vermicularis, Ascaris lumbricoides, Trichuris trichiura, Trichinella spiralis, Toxocara cati, Toxocara canis. Parasitic arthropods (Arthropoda), their significance as vectors. Insects, ticks etc.
|
Total ECTS (Creditpoints):
6.00
Contact hours:
63 Academic Hours
Final Examination:
Exam (Written)
Bibliography
Required Reading
1.
MIKROBIOLOĢIJA
2.
Medical Microbiology, Murray, Patrick R. Datu bāze:
3.
ClinicalKey
4.
Jawetz, Melnick & Adelberg’s Medical Microbiology
5.
AccessMedicine
6.
Review of Medical Microbiology and Immunology, Warren Levinson
7.
AccessMedicine
8.
Sherris Medical Microbiology, Ryan
9.
AccessMedicine
10.
Metodiskās rekomendācijas praktiskajām nodarbībām „Vispārīgā mikrobioloģija”. Rīga, RSU, 2015
11.
Imunoloģija:
12.
Review of Medical Microbiology and Immunology, Warren Levinson
13.
AccessMedicine
14.
Cellular and Molecular Immunology, Abbas Abul K.
15.
ClinicalKey
16.
Metodiskās rekomendācijas praktiskajām nodarbībām „Infekcija un imunitāte”. Rīga, RSU, 2017.
Additional Reading
1.
Prof. A.Lejnieka red. Klīniskā medicīna. 3. grāmata.Medicīnas apgāds. 2012.
2.
A. Žileviča, Medicīna mikrobioloģija I Vispārīgā mikrobioloģija un infekcijas imunoloģija, LU Akadēmiskais apgāds, 2014.
Other Information Sources
1.
http://estudijas. rsu.lv.