Veidlapa Nr. M-3 (8)
Study Course Description
Oxygen Derivatives and Biochemical Aspects of Free Radicals in Biology and Medicine
Course Code
SUUK_078
Branch of Science
Clinical medicine; Nutrition
ECTS
3.00
Target Audience
Medical Services; Public Health
LQF
Level 6
Study Type And Form
Full-Time; Part-Time
Study Course Implementer
Course Supervisor
Andrejs Šķesters
Structure Unit Manager
Liāna Deklava
Structural Unit
Department of Rehabilitation
Contacts
Riga, 3 Cigoriņu Street, spk@rsu.lv, +371 67611559
About Study Course
Objective
To provide in-depth knowledge on oxygen and nitrogen species biochemistry (active non-radical species of active oxygen and nitrogen) (nutrition aspect).
Preliminary Knowledge
Biochemistry, physiology.
Learning Outcomes
Knowledge
1.After successful completion of the study course the student will be able to: 1) To tell about the formation and activity of active forms of oxygen and nitrogen and radicals. 2) To define knowledge about antioxidants, necessary microelements, their role in the norm, changes in risk groups, as well as in cases of more common diseases and pathological conditions. 3) To understand the analytical equipment used in laboratory practice and to interpret modern, standardized analytical methods, to inform about the quality control of analyzes regulated by the existing legislation.
Skills
1.As a result of the course the student will apply the acquired theoretical knowledge in practice: Learning to explain: - Possibility of formation of oxidants in the body; - Changes in antioxidants and trace elements in risk groups, major diseases and pathologies; -Will be able to evaluate laboratory equipment and explain the results. -Will be able to evaluate quality control of regulatory analyzes.
Competences
1.Upon successful completion of this course, students will gain an understanding of free radical biochemistry, pro-oxidative and antioxidant processes in the body and their relationship to various pathological conditions and diseases, as well as prepare for independent research in radical biochemistry.
Assessment
Individual work
|
Title
|
% from total grade
|
Grade
|
|---|---|---|
|
1.
Individual work |
-
|
-
|
|
Attendance of lectures is free, participation in 2 seminars, practical works and laboratory works is obligatory.
The tasks of the study course are:
1) To give an idea of the active forms of oxygen and nitrogen and the formation and function of radicals;
2) To provide knowledge about antioxidants, the necessary trace elements, their role in the norm, changes in risk groups, as well as in cases of common diseases and pathological conditions;
3) To acquaint with the analytical equipment used in laboratory practice and modern, standardized analytical methods, as well as to inform about the quality control of analyzes regulated by the existing legislation.
|
||
Examination
|
Title
|
% from total grade
|
Grade
|
|---|---|---|
|
1.
Examination |
-
|
-
|
|
For obtaining credit points is required participation in all seminars (40%), laboratory work (15%) and practical work (15%), as well the answers to the final test question must be hand in (30%).
|
||
Study Course Theme Plan
FULL-TIME
Part 1
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Laboratory detection of some AO and / or LPO markers. BR and ASF markers are chosen depending on the students' basic specialization or interests. Many variants are possible here: for example - quantification of polyvinol, evaluation of antioxidant capacity using FRAP, CUPRAC, ABTS methods, evaluation of BR potency with DPPH, ORAC, HO-, determination of superoxide or using automatic analyzer, SOD, GPx
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
FR and ROS as toxic derivatives of biochemical reactions. Reactive species as useful biomolecules.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Oxidative stress and antioxidant protection: how does it work in practice? Detection of free radicals and some other ASF in the laboratory Use of natural antioxidants and bioactive substances in adjunctive therapy (polyphenols, carotenoids, tannins, anthocyanins, etc.). Mediterranean diet as an effective anti-stressor.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Free Radicals and antioxidant’s defence. Species with antioxidative and antiradical properties (RedOx homeostasys regulatory mechanism)
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Laboratory detection of some AO and / or LPO markers. BR and ASF markers are chosen depending on the students' basic specialization or interests. Many variants are possible here: for example - quantification of polyvinol, evaluation of antioxidant capacity using FRAP, CUPRAC, ABTS methods, evaluation of BR potency with DPPH, ORAC, HO-, determination of superoxide or using automatic analyzer, SOD, GPx
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
The chemistry of Free radicals (FR). Free radicals interrelation with ROS. FR, other “reactive species” and pathological conditions, and diseases.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Oxidative stress: adaptation, damage, repair and cell death. Types and conditions of origin of oxidative stress (OS). OS relationship with BR and ASF. Acute and chronic OS. Pathophysiological processes of OS (cardiovascular system, digestive organs, obesity, metabolic syndrome, type 2 diabetes and its complications - nephro-, neuro-, retinopathy)
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
FR and ROS as toxic derivatives of biochemical reactions. Reactive species as useful biomolecules.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
The chemistry of Free radicals (FR). Free radicals interrelation with ROS. FR, other “reactive species” and pathological conditions, and diseases.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Oxidative stress and antioxidant protection: how does it work in practice? Detection of free radicals and some other ASF in the laboratory Use of natural antioxidants and bioactive substances in adjunctive therapy (polyphenols, carotenoids, tannins, anthocyanins, etc.). Mediterranean diet as an effective anti-stressor.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Oxidative stress: adaptation, damage, repair and cell death. Types and conditions of origin of oxidative stress (OS). OS relationship with BR and ASF. Acute and chronic OS. Pathophysiological processes of OS (cardiovascular system, digestive organs, obesity, metabolic syndrome, type 2 diabetes and its complications - nephro-, neuro-, retinopathy)
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Oxygen as toxic gas. An introduction to oxygen toxicity and reactive oxygen species (ROS).
|
Total ECTS (Creditpoints):
3.00
Contact hours:
24 Academic Hours
Final Examination:
Exam
PART-TIME
Part 1
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Laboratory detection of some AO and / or LPO markers. BR and ASF markers are chosen depending on the students' basic specialization or interests. Many variants are possible here: for example - quantification of polyvinol, evaluation of antioxidant capacity using FRAP, CUPRAC, ABTS methods, evaluation of BR potency with DPPH, ORAC, HO-, determination of superoxide or using automatic analyzer, SOD, GPx
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Oxidative stress and antioxidant protection: how does it work in practice? Detection of free radicals and some other ASF in the laboratory Use of natural antioxidants and bioactive substances in adjunctive therapy (polyphenols, carotenoids, tannins, anthocyanins, etc.). Mediterranean diet as an effective anti-stressor.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Free Radicals and antioxidant’s defence. Species with antioxidative and antiradical properties (RedOx homeostasys regulatory mechanism)
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
The chemistry of Free radicals (FR). Free radicals interrelation with ROS. FR, other “reactive species” and pathological conditions, and diseases.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
FR and ROS as toxic derivatives of biochemical reactions. Reactive species as useful biomolecules.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Laboratory detection of some AO and / or LPO markers. BR and ASF markers are chosen depending on the students' basic specialization or interests. Many variants are possible here: for example - quantification of polyvinol, evaluation of antioxidant capacity using FRAP, CUPRAC, ABTS methods, evaluation of BR potency with DPPH, ORAC, HO-, determination of superoxide or using automatic analyzer, SOD, GPx
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
FR and ROS as toxic derivatives of biochemical reactions. Reactive species as useful biomolecules.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Oxidative stress: adaptation, damage, repair and cell death. Types and conditions of origin of oxidative stress (OS). OS relationship with BR and ASF. Acute and chronic OS. Pathophysiological processes of OS (cardiovascular system, digestive organs, obesity, metabolic syndrome, type 2 diabetes and its complications - nephro-, neuro-, retinopathy)
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Oxidative stress and antioxidant protection: how does it work in practice? Detection of free radicals and some other ASF in the laboratory Use of natural antioxidants and bioactive substances in adjunctive therapy (polyphenols, carotenoids, tannins, anthocyanins, etc.). Mediterranean diet as an effective anti-stressor.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Oxygen as toxic gas. An introduction to oxygen toxicity and reactive oxygen species (ROS).
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
Oxidative stress: adaptation, damage, repair and cell death. Types and conditions of origin of oxidative stress (OS). OS relationship with BR and ASF. Acute and chronic OS. Pathophysiological processes of OS (cardiovascular system, digestive organs, obesity, metabolic syndrome, type 2 diabetes and its complications - nephro-, neuro-, retinopathy)
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Other
|
2
|
Topics
|
The chemistry of Free radicals (FR). Free radicals interrelation with ROS. FR, other “reactive species” and pathological conditions, and diseases.
|
Total ECTS (Creditpoints):
3.00
Contact hours:
24 Academic Hours
Final Examination:
Exam
Bibliography
Required Reading
1.
Armstrong, D. eds. Free Radical and Antioxidant Protocols., pp.455. (1 eks. pie kursa autora) 1998. (akceptējams izdevums)
2.
Armstrong, D. eds. Free radicals in Diagnostic Medicine., pp.454.(1 eks. pie kursa autora)
3.
Dasgupta Amitava, Kimberly Klein. Antioxidants in Food, Vitamins and Supplements. 2016. Elsevier
4.
Vitamin E in Human Health. Peter Weber, Marc Birringer, Jeffrey B. Blumberg, Manfred Eggersdorfer, Jan Frank. Springer nature (2019)
5.
Integrative and Functional Medical Nutrition Therapy Principles and Practices. Diana Noland, Jeanne A. Drisko, Leigh Wagner. Springer nature (2020)
6.
Nutrition Guide for Physicians and Related Healthcare Professions. Editors (view affiliations) Ted Wilson, Norman J. Temple, George A. Bray. Springer nature (2022)
7.
Phenolic Antioxidants in Foods: Chemistry, Biochemistry and Analysis. Authors (view affiliations) Alam Zeb. Springer nature (2021)
Additional Reading
1.
Redox Biochemistry. Ruma Becker, Donald Dickman, Martin Banerjee. 2007. pp. 317.
2.
June H. Mcdermott. Antioxidant Nutrients: Current Dietary Recommendations and research update. J Am Pharm Assoc (Wash). 2000 Nov-Dec;40(6):785-99
3.
Peter H. Proctor, Edwards S. Reynolds. Free Radicals and Disease in Man. Physiol Chem Phys Med NMR. 1984;16(3):175-95.
4.
Tirzītis, G., Šķesters, A. Skābekļa atvasinājumu un brīvo radikāļu bioķīmiskie aspekti bioloģijā. Rīga, LU Akadēmiskais apgāds, 2007. 106 lpp. (LUB 30 gab.)
5.
Jākobsone, I. Pārtikas produktu uzturvērtības noteikšana. Rīga, LU Akadēmiskais apgāds, 2008. 152 lpp. (LUB 49 gab.)
6.
Selenius, O., Alloway, B., Centeno, J. A. et al. Essentials of Medical Geology. Impacts of the Natural Environment on Public Health. ELSEVIER Acad. Press. 2005., pp. 812.(1 eks. pie kursa autora)
Other Information Sources
1.
Journal „Free Radicals in Biology and Medicine"
2.
Journal „Free Radical Research”
3.
Journal „ European Journal of Clinical Nutritions”
4.
Journal „ Nutrition Journal”
5.
Dolph L. Hatfield. Selenium. Its Molecular Biology and Role in Human Health., pp. 326.