Veidlapa Nr. M-3 (8)
Study Course Description
Laboratory
Course Code
RNF_020
Branch of Science
Clinical medicine
ECTS
3.00
Target Audience
Medicine
LQF
Level 8
Study Course Implementer
Course Supervisor
Anna Popova
Residency Speciality
Speciality
Nephrologist
Supervisor of Medical Speciality
Anna Popova
Contacts
-
About Study Course
Objective
Provide medical residents with in-depth knowledge and practical skills in laboratory medicine, with a particular focus on clinical biochemistry, haematology, immunology, microbiology, immunohaematology and transplantation immunology; develop clinical thinking capabilities in the context of laboratory diagnostics and promote laboratory and physician collaboration, especially in the context of transplant examinations, immunosuppression monitoring and infection control.
Learning Outcomes
Knowledge
1.- basic principles of laboratory examinations: biochemistry, immunology, microbiology, immunohematology - major studies in transplant patients: HLA, DSA, infection diagnosis, immunosuppression monitoring. Basic principles, meaning of examinations. - Preanalytical, analytical and postanalytic steps and their effect on results - principles of laboratory quality control, standards requirements and accreditation process. - Laboratory information systems (LIS) and their connection to the hospital IT environment.
Skills
1.- interpret biochemical, haematological, immunological and microbiological data. - evaluate laboratory results in the context of transplantation: monitoring of recipient examinations, compatibility, post-transplantation - recognize false or inconsistent results
Competences
1.- understand the effects of preanalytical, analytical and postanalytic phases on laboratory results. - is able to evaluate the quality and relevance of laboratory examinations in a particular patient’s case - able to engage in clinical decision-making in the management of transplant patients based on laboratory data.
Assessment
Examination
|
Title
|
% from total grade
|
Grade
|
|---|---|---|
|
1.
Test |
-
|
10 points
|
|
Knowledge is assessed in a 10-point system. Assessment of practical skills in a 10-point system. |
||
Study Course Theme Plan
FULL-TIME
Part 1
-
Seminar
|
Modality
|
Location
|
|
|---|---|---|
|
On site
|
Study room
|
Topics
|
Laboratory role in monitoring transplant patients
• Importance of HLA compatibility in transplantation
• Donorspecific antibodies (DSA), PRA, cfDNA
• Individual compatibility (Crossmatch) tests and their interpretation
|
-
Seminar
|
Modality
|
Location
|
|
|---|---|---|
|
On site
|
Study room
|
Topics
|
Cooperation between laboratory and physician: from sample to decision
• Critical indicators, impact of errors
• Quality control and laboratory information system (LIS)
• Interpretation of results and comments to nephrologist
|
Total ECTS (Creditpoints):
3.00
Number of Residency Seminars:
2
Length (weeks):
2
Final Examination:
Residency exam (Theory and practice)
Bibliography
Required Reading
1.
Leber, A. L. (Ed.) (2024) The Clinical Microbiology Procedures Handbook, 5th edition. ASM Press
2.
Storoženko, J. (Ed.) (2024). Laboratoriskie izmeklējumi dažādu slimību diagnostikā. Medicīnas apgāds.
3.
Journal of Clinical Microbiology
4.
Richard A. McPherson, Matthew R. Pincus (2021) Henry’s Clinical Diagnosis and Management by Laboratory Methods, 24th edition.
5.
Tait BD. Transplantation Immunology: Methods and Protocols. Springer Protocols, 2021. (Praktisks pārskats par HLA tipēšanu, antivielu noteikšanu, crossmatch u.c.)
6.
Rabinovitch A., et al. Clinical Chemistry: Principles, Techniques, and Correlations. Lippincott Williams & Wilkins, 2020. (Plašs laboratorijas rokasgrāmatas formāts, īpaši bioķīmijā un hematoloģijā.)
7.
KDIGO Clinical Practice Guidelines for the Care of Kidney Transplant Recipients. Kidney Int Suppl, 2009 & jaunākie papildinājumi. (Vadlīnijas nefrologiem par transplantācijas pacientu aprūpi, tostarp imūnsupresijas monitoringu.)
8.
EFI Standards for Histocompatibility & Immunogenetics Laboratories. (Eiropas standarti HLA testēšanas laboratorijām; noder izpratnei par kvalitāti un metodēm.)
9.
HLA Guide (plaša brīvpieejas informācija, ASV)
10.
Mamode Nizam , Bestard Oriol , Claas Frans , Furian Lucrezia , Griffin Siân , Legendre Christophe , Pengel Liset , Naesens Maarten. European Guideline for the Management of Kidney Transplant Patients With HLA Antibodies: By the European Society for Organ Transplantation Working Group. Transplant International, Volume 35 – 2022, DOI=10.3389/ti.2022.10511
11.
STAR 2017 Working Group Meeting Report. Tambur AR, Lonjeroa G, et al. Sensitization in Transplantation: Assessment of Risk (STAR) 2017 Working Group Meeting Report. *Am J Transplant*. 2018;18(9):2120‑34.
12.
STAR 2019 Working Group Meeting Report. Tambur AR, Campbell P, Chong AS, et al. Sensitization in transplantation: Assessment of Risk 2019 Working Group Meeting Report. *Am J Transplant*. 2020;20(10):2652‑68.
13.
STAR 2022 Clinical Recommendations for Posttransplant DSA Assessment. Lefaucheur C, Louis K, Morris AB, et al.; STAR 2022 Working Group. Clinical recommendations for posttransplant assessment of anti‑HLA donor‑specific antibodies: A Sensitization in Transplantation: Assessment of Risk consensus document. *Am J Transplant*. 2023;23(1):115‑32.