Veidlapa Nr. M-3 (8)
Study Course Description
Molecular and Cell Biology
Course Code
BUMK_072
Branch of Science
Biological sciences; Environmental biotechnology; Medical biotechnology; Molecular Biology
ECTS
4.00
Target Audience
Biology; Medicine
LQF
Level 7
Study Type And Form
Full-Time
Study Course Implementer
Course Supervisor
Zanda Daneberga
Structure Unit Manager
Juta Kroiča
Structural Unit
Department of Biology and Microbiology
Contacts
Riga, Dzirciema Street 16, bmk@rsu.lv, +371 67061584
About Study Course
Objective
To provide students with the knowledge of molecular processes in the cell, as well as understanding of the role of human genome variation in aetiology of human pathology. To develop students’ skills in learning molecular biology laboratory methods, to help to understand the use of the acquired theoretical knowledge in medicine.
Preliminary Knowledge
Basic knowledge of biology and chemistry (in accordance with the standard for secondary education adopted by National Centre for Education).
Learning Outcomes
Knowledge
1.Upon completion of the study course, students will be able to explain the central dogma of molecular biology and its role in providing functions of eukaryotes and prokaryote cells. They will be familiar with the operational principles of the information realisation system, its regulation and functional role in eukaryotes and prokaryotes. Able to describe the main molecular mechanisms for ensuring normal cell functions as well as to justify the consequences of cellular disorders.
Skills
1.On completion of the study course, students will be able to use a light microscope, to plan and carry out experiments to monitor cell functions. Students will have acquired skills in molecular biology techniques – nucleic acid isolation and polymerase chain reaction, and they will be able to interpret the results obtained. Based on the analysis of scientific literature, students will be able to collect the most relevant information and correctly apply the concepts of molecular biology.
Competences
1.Students will be able to combine theoretical knowledge and skills and integrate them into learning of other preclinical and clinical courses. Students will be able to attribute cellular disorders to the pathology of the body as a whole. They will understand the role of structural and functional disorders of the genome in the aetiology of diseases.
Assessment
Individual work
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Title
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% from total grade
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Grade
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|---|---|---|
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1.
Individual work |
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-
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Individual work with the lecture and practical classes materials, recommended literature, according to the topic of lectures and practical classes. Analysis of scientific publications to acquire deeper understanding of the study course topics (if necessary). Upon completion the study course, fill in the study course evaluation questionnaire.
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Examination
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Title
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% from total grade
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Grade
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|---|---|---|
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1.
Examination |
-
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-
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Upon completion of the course “Molecular and Cell Biology”, students are obligated to take a final exam.
• The examination consists of multiple choice questions, short-answer questions, yes/no answers, matching answers, short open type questions.
• Students are eligible to receive an cumulative grade, thus, are exempt from taking the final exam, if they meet the following requirements:
- The student has taken all colloquia at their planned time for each group and passed successfully passed both colloquia, the average grade of each colloquium is at least 4.00 (if the colloquium is re-taken, the average grade is not mathematically rounded).
• The final cumulative grade is calculated taking into account grades received for colloquia (70%), practical classes protocols (25%) and online practical classes tests (5%).
• If exam has to be taken, the final grade is exam grade.
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Study Course Theme Plan
FULL-TIME
Part 1
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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Off site
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E-Studies platform
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2
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Topics
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Introduction to molecular biology. The central dogma of molecular biology. Eukaryotic and prokaryotic cells. Storage of information in the cell. Nucleic acids, their chemical composition, structure and functions. DNA replication.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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Off site
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E-Studies platform
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2
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Topics
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Nucleus and mitochondria, their structure and functions. Chromosomes, chromatin structure and types.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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Off site
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E-Studies platform
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2
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Topics
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Eukaryotic and prokaryotic genome – gene structure, organisation and functions. Genetic code.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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Off site
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E-Studies platform
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2
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Topics
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Transfer of genetic information in eukaryotic and prokaryotic cell – transcription, translation. Processing of proteins. Structure and functions of proteins.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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Off site
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E-Studies platform
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2
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Topics
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Human genome variation. Genetic variants and mutational process, its impact on human pathology.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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Off site
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E-Studies platform
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2
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Topics
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Molecular mechanisms of gene expression – epigenetic and epigenomic regulation.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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Off site
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E-Studies platform
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2
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Topics
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Cytoskeleton, its structure and functions. Cell division of eukaryotic cell – mitosis and meiosis. Cell division in prokaryotes. Recombination of genetic material.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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Off site
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E-Studies platform
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2
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Topics
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The cell cycle, regulation mechanisms. DNA reparation mechanisms. Molecular mechanisms of cell aging and cell death.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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Off site
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E-Studies platform
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2
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Topics
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Molecular mechanisms of gametogenesis and fertilisation, impact on development of human pathology.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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Off site
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E-Studies platform
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2
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Topics
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Molecular mechanisms of cell functions. Intracellular transport. Transmembrane transport of small molecules, ions and macromolecules. Endomembrane system, its structure and functions.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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Off site
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E-Studies platform
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2
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Topics
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Molecular mechanisms of cell functions – cell communication. Intracellular and extracellular signal transductions. Extracellular matrix, its structure and functions.
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Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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Off site
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E-Studies platform
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2
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Topics
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Molecular biology in vitro methods in medicine.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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2
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Topics
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Practical class No. 1. The nucleus and DNA of eukaryotes. DNA replication.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Other
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2
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Topics
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Practical class No. 2. DNA replication in vitro - polymerase chain reaction.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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2
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Topics
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Practical class No. 3. Human metaphase chromosomes in microscope slides. Karyotype.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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2
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Topics
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Practical class No. 4. Tasks of molecular biology.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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2
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Topics
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Practical class No. 5. Epigenetics vs. epigenomics. Tasks in epigenetics.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Computer room
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2
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Topics
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Colloquium I. Testing knowledge of topics covered during lectures No. 1 – 6, and practical classes No. 1 – 5.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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2
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Topics
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Practical class No. 6. Identification of stages in karyokinesis (microscopy slides).
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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2
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Topics
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Practical class No.7. Analysis of stages in gametogenesis, mature sex cells (microscopy slides).
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Other
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2
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Topics
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Practical class No. 8. Transport of solutes in the eukaryotic cell.
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Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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2
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Topics
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Practical class No. 9. Cells signalling systems. Analysis of different signalling pathways.
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-
Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Other
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2
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Topics
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Practical class No. 10. Molecular biology in vitro methods in medicine – case studies.
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-
Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Computer room
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2
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Topics
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Colloquium II. Testing knowledge of topics covered during lectures No. 7 – 12 and practical classes No. 6 -10.
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Total ECTS (Creditpoints):
4.00
Contact hours:
48 Academic Hours
Final Examination:
Exam (Written)
Bibliography
Required Reading
1.
Krūmiņa A., Baumanis V. Eikariotu šūnu bioloģija. Rīga, RSU, 2015. (latviešu plūsmas studijām /for studies in Latvian)
2.
Pollard T. D., Earnshaw W. C. – Cell Biology. 4th ed., Saunders, Elsevier, 2023.Suitable for English stream
3.
Karp's Cell and Molecular Biology. John Wiley & Sons; 8th Edition, Global edition, 2020.Suitable for English stream
Additional Reading
1.
Cooper G.M., Hausman R.E.- The cell. A Molecular approach. 8th ed., Oxford University press, 2023.Suitable for English stream
2.
Bruce A., et al.- Molecular biology of the cell 6th edition or later. New York, NY: Garland Science, Taylor and Francis Group, 2022.Suitable for English stream
3.
Lodish H, Berk A, Zipursky SL, et al. Molecular Cell Biology. 9th edition. New York: W. H. Freeman; 2021.Suitable for English stream
Other Information Sources
1.
Internet. NCBI (DNA &RNA, Genetics&Medicine, Genes &Expression, Genomes&Maps).Suitable for English stream