Forensic Chemistry and Toxicology

to provide theoretical knowledge of forensic toxicology and acquire practical skills working with material evidence (biological samples) and collection of samples in accordance with the Cabinet of Ministers Regulations. No. 268 (entered into force on 06.01.2016.) “Regulations for medical staff and students who acquire the first or second level professional higher education about the expertise in treatment, and the amount of their practical and theoretical knowledge” 5. Content of the programme: training includes mastering basic knowledge, skills and gaining experience about: laboratory work specifics in forensic toxicology, detection of toxic substances in biologic matherial (blood, urine, sweat, hair, content of digestive tract, organ’s tissues) and into material evidence, collection and packaging of biological evidence; extraction from various biological objects; Theoretical education: seminars 21. Poisoning with metals, corrosive substances, technical liquids. D. Tribis 22. Toxic and lethal concentrations. Metabolites, their determination. Interpretation of death from poisoning. D. Tribis Practical studies Nr. Theme Resp. 1. Special requirements for medical laboratories. LVS EN ISO/IEC 17025 standard demands. Recommendations of the International Association of Forensic Toxicologists (TIAFT) and United Nations Office on Drugs and Crime (UNODC). Dainis Tribis 2. Classical and modern toxic substances’ isolation methods: extraction, distillation, deflegmation, dialysis, mineralization and destruction. Cadaveric material rotting products impact on poisons detection methods. Dainis Tribis 3. Narcotic and psychotropic substances. Classification. Detection methods in seized materials and biological specimens. Opiates and opioids. Cocaine and its metabolites. Amphetamine and its derivatives (MDA, MDMA, MDEA, MBDB, ephedrine, ephedrone, methamphetamine). New psychoactive substances (NPS). Cannabis products. Pharmacognostical investigation of cannabis herbal products. Psilocin and psilocybin. LSD. Mescaline. Fentanyl derivatives. Derivatives of benzodiazepine and dibenzoazepine. Barbiturates and thiobarbiturates. Derivatives of phenothiazine and thioxanthene. Derivatives of butyrophenone, imidazoline. Oxybutyrate and butyrolactone. Derivatives of pyrazolone. Salicylates. Tricyclic antidepressants. Zolpidem, zopiclon, zaleplon. Combined poisonings. Dainis Tribis 4. Drugs screening by enzyme multiplied immunoassay technique (EMIT), gas chromatography-masspectrometry. Thin layer chromatography. Sample preparation and purification: liquid and solid phase extraction, gel-chromatography, re-extraction. Dainis Tribis 5. Ethyl alcohol and other volatile substances. Vidmark formula. Ethylene glycol and other glycols, cellosolves. Aromatic hydrocarbons. Isolation and gas chromatography analysis methods for volatile compounds. Dainis Tribis 6. Toxic gases. Carbon monoxide. Carboxyhemoglobin and carboxymyoglobin. Methods for the determination of carboxyhemoglobin. Analysis of other toxic gases. Dainis Tribis 7. Toxicology of cardiac glycosides. Isolation methods. Identification and quantitation methods. Calcium antagonists. Beta-blockers. Dainis Tribis 8. Tear gases (CS, CN, chloropicrin). Analysis methods (gas chromatography). Dainis Tribis 9. Poisonous plants and mushrooms. Animal and fish toxins. Snake and bee venom Sabotage poisons. Dainis Tribis 10. Heavy metals and metalloids. Toxicology and analysis methods: x-ray fluorescence spectroscopy, atomic absorption spectroscopy (AAS). Dainis Tribis 11. Analysis of cations and anions. Concentrated acids and bases. Acetic acid analysis. Dainis Tribis 12. Organophosphate poisoning, analysis methods. Pesticides. Classification. Analysis methods. Dainis Tribis 13. Analysis of household chemicals. Dainis Tribis 14. Unknown substance analysis. Dainis Tribis 15. Drivers and other persons alcohol and drug test. Breathalyzers. Results interpretation. Dainis Tribis 16. Micro-crystalloscopy and crystal optics. Application in drug analysis. Dainis Tribis 17. Modern chromatography methods (HPLC, GC-MS, LC-MS, capillary electrophoresis etc.). Derivatization. Technique and reagents. Dainis Tribis 19 Toxicokinetics and toxicodynamics. Analysis results interpretation. Post mortem drug redistribution. Therapeutic, toxic, lethal concentrations. Sources of toxicology information. Bibliography, data bases. Dainis Tribis Acquired manipulutions – taking, packing and delivering samples from 100 examinations of corpses during 3rd, (4th) years studies

examination of theoretical knowledge with multiple choice test, examination of practical skills with situational task. Evaluation of results in 10 grade system.