Veidlapa Nr. M-3 (8)
Study Course Description
Immunophenotyping by Flow Cytometry
Course Code
RHE_051
Branch of Science
Clinical medicine
ECTS
9.00
Target Audience
Medicine
LQF
Level 8
Study Course Implementer
Course Supervisor
Sandra Lejniece
Residency Speciality
Speciality
Hematologist
Supervisor of Medical Speciality
Sandra Lejniece
Contacts
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About Study Course
Objective
To ensure acquisition and improvement of theoretical knowledge and practical skills in immunophenotyping by flow cytometry in accordance with the requirements of the haematology specialty in order to prepare the doctor for certification in the haematology specialty.
Learning Outcomes
Knowledge
1.- Will know about the flow cytometry of blood, bone marrow and other body fluids; - Will know about cell markers in the diagnosis of haematological diseases; - Will know about the application of flow cytometry in the diagnosis of acute and chronic leukaemias and lymphomas; - Will know about the application of flow cytometry in stem cell collection, during autologous and allogeneic transplantation..
Skills
1.- Able to analyse the results of flow cytometry of blood, bone marrow and other body fluids.
Competences
1.Analysis of flow cytometry results of blood, bone marrow and other body fluids.
Assessment
Individual work
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Title
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% from total grade
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Grade
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1.
Individual work |
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1. Independently studies the literature related to the course topics;
2. Independently prepares for seminars;
3. Gets acquainted with and analyses medical documentation;
4. Completes medical documentation;
5. Independently follows the instructions of the certified specialist.
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Examination
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Title
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% from total grade
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Grade
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|---|---|---|
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1.
Examination |
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-
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Mandatory attendance of seminars (100%) and performance of practical work (100%) in each study course.
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Study Course Theme Plan
FULL-TIME
Part 1
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Seminar
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Modality
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Location
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Topics
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Pre-analytical and analytical phase of the flow cytometry of blood, bone marrow and other body fluids.
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Seminar
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Modality
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Location
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Topics
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The most important or essential cell markers in the diagnosis of haematological diseases. Cell line-specific, cell progenitor and differentiation markers.
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Seminar
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Modality
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Location
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-
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Topics
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Common principles for building disease-targeted antibody panels.
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Seminar
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Modality
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Location
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Topics
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Post-analytical phase of flow cytometry (data analysis, diagnosis of cell lines, determination of specific subtypes).
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Seminar
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Modality
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Location
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|---|---|---|
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Topics
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Application of flow cytometry in the diagnosis of acute and chronic leukaemias and lymphomas, limitations and prognostic significance. The concept of minimal residual disease.
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Seminar
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Modality
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Location
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|---|---|---|
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-
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Topics
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Application of flow cytometry in stem cell collection, during autologous and allogeneic transplantation.
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Total ECTS (Creditpoints):
9.00
Number of Residency Seminars:
6
Length (weeks):
7
Final Examination:
Residency exam (Theory and practice)
Bibliography
Required Reading
1.
Marion G.Macey. Flow Cytometry: Principles and Applications. Human press, 2019
2.
Cristine Goetz, Cristopher Hammerbeck, Jody Bonnevier. Flow Cytometry Basics for the Non-Expert. Springer, 2017
3.
International Agency for Research on Cancer (Author), S.H. Swerdlow. WHO classification of tumours of haematopoietic and lymphoid. Vol. 2 Paperback – 18 Sept. 2017.
4.
Alla Rivkina. Plūsmas citometrijas metode hematoloģisko slimību diagnostikā. SIA Medicīnas apgāds, 2019.
5.
Arber DA, Orazi A, Hasserjian R, et al. The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia. Blood 2016; 127:2391-2405. https://doi.org/10.1182/blood-2016-03-643544
6.
Emile JF, Abla O, Fraitag S, et al. Revised classification of histiocytoses and neoplasms of the macrophage-dendritic cell lineages. Blood 2016; 127:2672
7.
S.Lejniece. Klīniskā hematoloģija. Medicīnas Apgāds, 2020
8.
Klīnisko analīžu rokasgrāmata. E.Gulbja laboratorija. Izdevējs E.Gulbja laboratorija, 2019.
Additional Reading
1.
Richard A. McPherson MD, MSc, Matthew R. Pincus MD, PhD, Matthew R. Pincus. Henry's Clinical Diagnosis and Management by Laboratory Methods / Edition 21. Saundrs, 2006.
2.
Leslie E. Silberstein, MD, John Anastasi, MD. Hematology: Basic Principles and Practice E-Book. Elsevier, 2007.
3.
Berger, D.P., Engelhardt, M., Henß, H., Mertelsmann, R., Andreeff, M., Koziner, B., Messner, H.A., Thatcher, N. (Eds.). Concise Manual of Hematology and Oncology, 2008.