Veidlapa Nr. M-3 (8)
Study Course Description
Virology and Molecular Biology
Course Code
RLA_027
Branch of Science
Clinical medicine; Microbiology and Virology
ECTS
27.00
Target Audience
Medicine
LQF
Level 8
Study Course Implementer
Course Supervisor
Jeļena Storoženko
Residency Speciality
Speciality
Laboratory doctor
Supervisor of Medical Speciality
Jeļena Storoženko
Contacts
-
About Study Course
Objective
To prepare laboratory doctors capable of performing laboratory diagnostics (serological, molecular biological, virus isolation) of infectious diseases caused by viruses, capable of using molecular biological diagnostics for diagnostics of diseases caused by bacteria and viruses. To understand the significance and role of epidemiological examinations in identification of the source of infection. To use examination algorithms based on international guidelines and recommendations, to interpret the obtained results. To master internal and external quality control methods.
Learning Outcomes
Knowledge
1.- to evaluate advantages of different virological and molecular biological methods; - to describe principles of diagnostic methods used in virology; - to characterise the creation of a diagnostic algorithm of viral infections; - to evaluate laboratory diagnostics and treatment monitoring of viral hepatitis and HIV/AIDS; - to describe rules of sampling of biological materials to perform virological and molecular biological examinations..
Skills
1.- to prepare samples for virological and molecular biological examination; - to use manual and automated examination methods; - to perform isolation of viruses and indication of viruses in a clinical material; - to perform identification of RNA/DNA of viruses and bacteria; - to conduct immunochemical examination methods to determine antibodies and antigens.
Competences
1.To perform diagnostic examinations of human biological material for diagnostics of viral infections. Based on exercise results to perform diagnostics, differential diagnostics of diseases, to interpret results and to recommend further examination activity to clinicians.
Assessment
Individual work
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Title
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% from total grade
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Grade
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|---|---|---|
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1.
Individual work |
-
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-
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Preparation of a presentation about the epidemiological HIV monitoring system and laboratory diagnostics principles in Latvia.
In-depth research: cultivation of viruses, monitoring of virus circulation, use of virus genotyping methods.
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Examination
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Title
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% from total grade
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Grade
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|---|---|---|
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1.
Examination |
-
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-
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2.
Examination |
-
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-
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Assessment of knowledge – written answers to questions at the end of the study course.
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Study Course Theme Plan
FULL-TIME
Part 1
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Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Serological methods: immunochemical bases, types, characteristics, quality control
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-
Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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HIV epidemiological surveillance system in Latvia, tasks of testing laboratories in its implementation
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-
Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Basics of HIV / AIDS laboratory diagnostics, laboratory treatment monitoring
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-
Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Basics of laboratory diagnosis of AIDS opportunistic infections
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-
Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Laboratory diagnosis of liver diseases caused by infectious agents
Laboratory diagnosis of liver diseases caused by infectious agents
Laboratory diagnosis of liver diseases caused by infectious agents
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Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Laboratory diagnostics of viral hepatitis, laboratory monitoring of treatment.
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-
Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Diagnosis of tick-borne infectious diseases
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Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Virological methods in the diagnosis of infectious diseases.
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Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Diagnosis of respiratory viral infections
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-
Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Diagnosis of viral gastroenteritis
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Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Diagnosis of enterovirus infections
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Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Classification of molecular biological methods, advantages and limitations of their application
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Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Molecular biological epidemiology
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Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Molecular biological methods in bacteriology and virology
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-
Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Interpretation of laboratory test results and development of algorithms
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-
Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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Legislation on communicable disease surveillance. The role of laboratories in the surveillance of infectious diseases.
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Seminar
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Modality
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Location
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|---|---|---|
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On site
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Auditorium
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Topics
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The level of biosafety in a medical laboratory. Diagnosis of dangerous infectious diseases
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Total ECTS (Creditpoints):
27.00
Number of Residency Seminars:
17
Length (weeks):
20
Final Examination:
Residency exam (Theory and practice)
Bibliography
Required Reading
1.
Barbara Detrick and Robert G. Hamilton. Manual of Molecular and Clinical Laboratory Immunology. 8th Edition, 2016.
2.
Lynn B. Jorde, John C. Carey, Michael J. Medical Genetics. 5th Edition, 2016
3.
Mary Louise Turgeon. Immunology and Serology in Laboratory Medicine. 7th Edition, 04/2021
4.
Diagnostic Methods in Virology. Virological Methods. Virus Culture
Additional Reading
1.
Methods Slideset
2.
Real-time PCR handbook, lifetechnologies.com
4.
Beginner’s Guide to Real-Time PCR, Primerdisign, vers.4.3.1
5.
Wang-Shick Ryu. Molecular Virology of Human Pathogenic Viruses. 2017
Other Information Sources
1.
http://www.ebook3000.com/Leslie-Collier--John-Oxfordquot-Human-Virology_104234.html Leslie Collier, John Oxford quot; Human Virology, 2010 .
3.
Diagnostic Methods in Virology, Virological Methods, Virus Culture
5.
Virological Methods Slideset
6.
Microbiology. 4th edition. Edited by Samuel Baron. 4th edition. University of Texas Medical Branch at Galveston, Galveston, Texas Galveston (TX): University of Texas Medical Branch at Galveston; 5. http://ideas.health.vic.gov.au/bluebook.asp
7.
Infectious Diseases Epidemiology & Surveillance
8.
Infectious Diseases Epidemiology & Surveillance. The American Journal of the Medical Sciences. Editor-in-Chief: David W. Ploth. http://ideas.health.vic.gov.au/bluebook.asp