Veidlapa Nr. M-3 (8)
Study Course Description
Molecular Biological Examination Methods
Course Code
RKM_022
Branch of Science
Clinical medicine
ECTS
16.00
Target Audience
Medicine
LQF
Level 8
Study Course Implementer
Course Supervisor
Aigars Reinis
Residency Speciality
Speciality
Clinical microbiologist
Supervisor of Medical Speciality
Aigars Reinis
Contacts
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About Study Course
Objective
To provide knowledge and practical skills in molecular biological examination methods used in microbiology.
Learning Outcomes
Knowledge
1.To describe the different technologies for nucleic acid detection and the detection and analysis of post-amplification products.
Skills
1.To apply practical molecular biology methods commonly used in laboratories and analyse the results.
Competences
1.In collaboration with clinicians, to choose the most effective molecular biology method to achieve a diagnostic result.
Assessment
Individual work
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Title
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% from total grade
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Grade
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|---|---|---|
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1.
Individual work |
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-
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To continuously and thoroughly learn the principles of molecular biology technologies and prepare presentations on them at seminars.
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Examination
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Title
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% from total grade
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Grade
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|---|---|---|
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1.
Examination |
-
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-
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Participation in seminars and practical work, assessed on a 10-point scale, which accounts for 50% of the final assessment, exam - 50%.
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Study Course Theme Plan
FULL-TIME
Part 1
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Seminar
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Modality
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Location
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Topics
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Selection of correct material and transport media, transportation and storage of material.
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Seminar
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Modality
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Location
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-
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Topics
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Probe-based nucleic acid detection technologies without amplification (hybridisation).
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Seminar
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Modality
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Location
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Topics
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Technologies for the detection of nucleic acids by amplification: Signal amplification methods (DNA/RNA hybrid capture with monoclonal antibodies and fluorescent signal detection, Šķēlēja-Invader technology (patented)).
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Seminar
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Modality
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Location
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Topics
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Nucleic acid detection methods with amplification - amplification methods for target nucleic acids: Polymerase chain reaction.
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Seminar
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Modality
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Location
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Topics
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Reverse transcriptase polymerase chain reaction.
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Seminar
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Modality
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Location
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Topics
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Nested polymerase chain reaction.
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Seminar
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Modality
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Location
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Topics
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A two- or three-step polymerase chain reaction aimed at reducing amplification of non-specific products.
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Seminar
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Modality
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Location
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Topics
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Multiplex PCR.
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Seminar
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Modality
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Location
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Topics
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Polymerase chain reaction with real-time signal detection.
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Seminar
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Modality
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Location
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Topics
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Transcription-based amplification methods.
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Seminar
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Modality
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Location
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Topics
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Isothermal amplification methods: strand displacement amplification, loop-mediated amplification, helicase-dependent amplification.
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Seminar
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Modality
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Location
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Topics
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Post-amplification product detection and analysis options: gel electrophoresis, capillary electrophoresis, colorimetric microplate reader system, sequencing, seeding of propagated material on microcellulose, strips or beads and its detection, mass spectrometry.
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Total ECTS (Creditpoints):
16.00
Number of Residency Seminars:
12
Length (weeks):
12
Final Examination:
Residency exam (Theory and practice)
Bibliography
Required Reading
1.
Clinical Microbiology Procedures Handbook. 4th Edition, 2016
2.
Laboratory Diagnosis. In: Levinson W., Chin-Hong P., Joyce E. A., Nussbaum J., Schwartz B. eds. Review of Medical Microbiology & Immunology: A Guide to Clinical Infectious Diseases. 16th Edition. McGraw-Hill; Accessed August 17, 2020.
3.
Principles of diagnostic medical microbiology. Eds. Riedel S., Hobden J.A., Miller S., et.al. Jawetz, Melnick, & Adelberg's Medical Microbiology, 28th Edition. McGraw-Hill, 2019.
4.
Manual of Molecular and Clinical Laboratory Immunology. 8th Edition, 2016
5.
Manual of Commercial Methods in Clinical Microbiology. John Wiley & Sons, Incorporated, 2016. ProQuest Ebook Central
6.
Molecular Medical Microbiology. edited by Andrew Sails, et al. Elsevier Science & Technology, 2014. ProQuest Ebook Central
Other Information Sources
1.
ClinicalKey
2.
PubMed
3.
AccessMedicine
4.
ScienceDirect