Veidlapa Nr. M-3 (8)
Study Course Description
Toxicological Chemistry
Course Code
LFK_048
Branch of Science
Basic medicine
ECTS
5.00
Target Audience
Pharmacy
LQF
Level 7
Study Type And Form
Full-Time; Part-Time
Study Course Implementer
Course Supervisor
Katrīna Sudmale
Structure Unit Manager
Baiba Mauriņa
Structural Unit
Department of Applied Pharmacy
Contacts
Riga, 21 Consula Street, farmkk@rsu.lv, +371 67061544
About Study Course
Objective
• to promote the acquisition of knowledge on the main methods of analysis for the determination of toxic substances in biological objects • to promote awareness of the possibilities of chemical and physico-chemical analysis methods in toxicological analysis • to master the types of tasks of the issues included in the course and the methodology of their solution
Preliminary Knowledge
Inorganic, organic and analytical chemistry, human anatomy, physiology and pathology.
Learning Outcomes
Knowledge
1.After completing the study course, students will be able to: • evaluate the objects to be used in the toxicological analysis; • present the theoretical basis of the methods used in the analysis; • understand and interpret analytical methodologies available in the literature.
Skills
1.As a result of mastering the study course students: • will be able to isolate different types (volatile, organic, non-volatile, metal-containing) toxicants from biological objects; • will be able to use chromatographic, spectroscopic methods and chromogenic reactions in an experiment to detect the most well-known toxicants; • will be able to apply toxicokinetics and toxicodynamics to the interpretation of the results of legality analyses.
Competences
1.As a result of the study course, students will be able to isolate the most well-known toxicants from biological objects, analyse and interpret the results.
Assessment
Individual work
|
Title
|
% from total grade
|
Grade
|
|---|---|---|
|
1.
Individual work |
-
|
-
|
|
Individual work in the laboratory, performing experiments, solving situation tasks. It is recommended to fill in the study course survey, evaluating the existing course content and creating proposals for updating the course.
|
||
Examination
|
Title
|
% from total grade
|
Grade
|
|---|---|---|
|
1.
Examination |
-
|
-
|
|
1. Colloquia and seminars on toxic effects, their isolation, analysis, data interpretation. (30%)
2. Solving situation tasks. (10%)
3. Practical isolation of toxicants from biological objects, their analysis and interpretation of results. (10%)
4. Exam: knowledge of the mechanisms of action of toxicants, their isolation from the section material, qualitative and quantitative analysis, the use of regularities of toxicokinetics and toxicodynamics in interpreting the results of analyses are tested. (50%)
|
||
Study Course Theme Plan
FULL-TIME
Part 1
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Toxicology and analytical toxicology. Dose-response relationship.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Analytical toxicology of volatile toxicants.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Physio-chemical properties and methods of isolation of non-volatile organic toxicants.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Chromatographic and spectral methods in analysis of drugs and other organic toxicants.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Analytical toxicology of toxic cations, anions and pesticides.
|
|
Essentials of toxicokinetics and toxicodynamics for interpretation of analytical results.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
2
|
Topics
|
Identification of non-volatile organic toxic substances.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Colloquium. General principles of analytical toxicology. Volatile toxicants.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Isolation and identification of volatile toxicants.
Analysis of volatile toxicants.
|
|
Isolation of non volatile organic toxicants.
Analysis of drugs in biological samples.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Colloquium.
Isolation and analysis of non-volatile organic toxicants.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Colloquium.
Chemical-toxicological analysis of poisonous cations, anions and pesticides.
|
|
Control analysis: Isolation and demonstration of drug substances.
|
|
Control analysis: isolation and detection of toxic cations.
|
|
Colloquium: Isolation and identification of toxicants.
|
Total ECTS (Creditpoints):
2.00
Contact hours:
24 Academic Hours
Final Examination:
Test (Semester)
Part 2
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Control analysis: Isolation and demonstration of volatile compounds. Full forensic chemical analysis.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Detection of toxic compounds in urine by colour reactions. Control analysis: Express methods in the diagnosis of poisoning.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Detection of drug substances in urine by solid phase extraction and thin layer chromatography screening.
Control test: Identification of the toxic compound.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Detection of therapeutic substances in urine by extraction and electron spectroscopy.
Control analysis: Identification of toxicants in urine by their UV spectra.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
3
|
Topics
|
Use of infrared spectroscopy for identification of toxicants.
Control analysis: Identification of drug substances by IS spectra.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Study room
|
3
|
Topics
|
Isolation and analysis of volatile toxic compounds. Control analysis (volatile poisons).
|
|
Isolation of non-volatile organic toxicants.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Study room
|
3
|
Topics
|
Identification of non-volatile organic toxicants. Control analysis (non-volatile organic poisons).
|
|
Isolation and demonstration of non-volatile organic toxicants.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Study room
|
3
|
Topics
|
Detection of toxic cations in minerals. Control analysis (metallic poisons).
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Study room
|
3
|
Topics
|
Detection of toxic anions. Control analysis.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Study room
|
3
|
Topics
|
Seminar on toxicokinetics.
Test on toxicokinetics.
|
|
Seminar on toxicant metabolism.
Test on metabolism.
|
|
Colloquium: Toxicokinetics and toxicodynamics.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Study room
|
3
|
Topics
|
Seminar on toxicokinetics.
Test on toxicokinetics.
|
|
Seminar on toxicant metabolism.
Test on metabolism.
|
|
Colloquium: Toxicokinetics and toxicodynamics.
|
Total ECTS (Creditpoints):
3.00
Contact hours:
33 Academic Hours
Final Examination:
Exam (Written)
PART-TIME
Part 1
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
4
|
Topics
|
Toxicology and analytical toxicology. Dose-response relationship.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
4
|
Topics
|
Analytical toxicology of volatile toxicants.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
4
|
Topics
|
Physio-chemical properties and methods of isolation of non-volatile organic toxicants.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
4
|
Topics
|
Chromatographic and spectral methods in analysis of drugs and other organic toxicants.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
4
|
Topics
|
Analytical toxicology of toxic cations, anions and pesticides.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
4
|
Topics
|
Essentials of toxicokinetics and toxicodynamics for interpretation of analytical results.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
4
|
Topics
|
Mineralization of biological material.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
4
|
Topics
|
Isolation and analysis of volatile toxic compounds. Control analysis (volatile poisons).
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
4
|
Topics
|
Isolation of non-volatile organic toxicants.
|
|
Identification of non-volatile organic toxicants. Control analysis (non-volatile organic poisons).
|
|
Isolation and demonstration of non-volatile organic toxicants.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
4
|
Topics
|
Detection of toxic cations in minerals. Control analysis (metallic poisons).
|
|
Detection of toxic anions. Control analysis.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
4
|
Topics
|
Control analysis: Isolation and demonstration of volatile compounds. Full forensic chemical analysis.
|
|
Control analysis: Isolation and demonstration of drug substances.
|
|
Control analysis: isolation and detection of toxic cations.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
4
|
Topics
|
Colloquium: Isolation and identification of toxicants.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
4
|
Topics
|
Detection of toxic compounds in urine by colour reactions. Control analysis: Express methods in the diagnosis of poisoning.
|
|
Detection of drug substances in urine by solid phase extraction and thin layer chromatography screening.
Control test: Identification of the toxic compound.
|
|
Detection of therapeutic substances in urine by extraction and electron spectroscopy.
Control analysis: Identification of toxicants in urine by their UV spectra.
|
-
Class/Seminar
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Laboratory
|
4
|
Topics
|
Seminar on toxicokinetics.
Test on toxicokinetics.
|
|
Seminar on toxicant metabolism.
Test on metabolism.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
4
|
Topics
|
Colloquium: Toxicokinetics and toxicodynamics.
|
-
Lecture
|
Modality
|
Location
|
Contact hours
|
|---|---|---|
|
On site
|
Auditorium
|
4
|
Topics
|
Colloquium: Toxicokinetics and toxicodynamics.
|
Total ECTS (Creditpoints):
5.00
Contact hours:
64 Academic Hours
Final Examination:
Exam (Written)
Bibliography
Required Reading
1.
Casarett and Doull's toxicology: the basic science of poisons / editor, Curtis D. Klaassen. 9th ed. New York: McGraw-Hill Education, 2019. xiii, 1454 lpp.Suitable for English stream
2.
V.Liguts. Toksikoloģijas rokasgrāmata. Rīga, 2001. (akceptējams izdevums)
3.
S.Jickels, A.Negrusz (eds). Clarke’s Analytical Forensic Toxicology. 2nd ed. Pharmaceutical Press, London, Chicago, 2013. (akcpetējams izdevums)Suitable for English stream
4.
R.J. Flanagan, et al. Fundamentals of Analytical Toxicology. Willey, 2020.Suitable for English stream
Additional Reading
1.
J.A.Timbrell. Introduction to Toxicology. London, 2023.Suitable for English stream
2.
A. C. Moffat, et al. (eds). Clarke’s Analysis of Drugs and Poisons. London, Chicago, 2011.Suitable for English stream