Veidlapa Nr. M-3 (8)
Study Course Description
Biochemistry II
Course Code
CFUBK_013
Branch of Science
Clinical medicine
ECTS
6.00
Target Audience
Life Science
LQF
Level 7
Study Type And Form
Full-Time
Study Course Implementer
Course Supervisor
Andrejs Šķesters
Structure Unit Manager
Pēteris Tretjakovs
Structural Unit
Department of Human Physiology and Biochemistry
Contacts
Riga, 16 Dzirciema Street, cfbk@rsu.lv, +371 67061550
About Study Course
Objective
To provide knowledge of the biochemistry of oxygen and nitrogen derivatives (active oxygen and nitrogen of non-radical nature and radicals).
Preliminary Knowledge
Medical Biochemistry and Normal Physiology RSU MF II course level.
Learning Outcomes
Knowledge
1.Upon successful completion of the study course, the student will be able to: 1) Tell about the formation and activity of active oxygen and nitrogen species and radicals. 2) Define knowledge of antioxidants, essential trace elements, their role in the norm, changes in risk groups, and in more common diseases and pathological conditions. 3) Understand the analytical equipment used in laboratory practice and interpret modern, standardised analytical methods, inform about the quality control of analyses regulated by existing legislation.
Skills
1.Having mastered the study course, the student will apply the acquired theoretical knowledge in practice: They will be able to explain: • The formation of oxidants in the body; • Changes in antioxidants and trace elements in risk groups, in cases of common diseases and pathologies. • The student will be able to evaluate the equipment used in laboratory practice and explain the obtained results. • The student will be able to evaluate the quality control of analyses regulated by legislation.
Competences
1.On successful completion of this course, students will understand free radical biochemistry, pro-oxidative and antioxidative processes in the body, and the links between these processes and various pathological conditions and diseases, and will be prepared for independent research work in the radical biochemistry.
Assessment
Individual work
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Title
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% from total grade
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Grade
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|---|---|---|
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1.
Individual work |
-
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-
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Independent study of theoretical topics using other sources, such as scientific books, publications.
In order to evaluate the quality of the study course as a whole, the student must fill out the study course evaluation questionnaire on the Student Portal.
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Examination
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Title
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% from total grade
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Grade
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|---|---|---|
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1.
Examination |
-
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-
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Participation in practical lessons, solving tasks.
In the final exam, a scientific work, using the acquired knowledge, on problem studies.
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Study Course Theme Plan
FULL-TIME
Part 1
-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Auditorium
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2
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Topics
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The concept of O2 as a toxic gas. Introduction to O2 toxicity and active oxygen species (AOS).
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-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Auditorium
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2
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Topics
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Free radical chemistry. Free radical chemistry in relation to AOS. Free radical chemistry, ASF and their relationship with the metabolic processes in the body.
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-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Auditorium
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2
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Topics
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Free radicals, other active forms (nitrogen radicals), as derivatives of biochemical processes in the body. AOS as biomolecules.
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-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
|
Auditorium
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2
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Topics
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Free radicals, active forms of oxygen and nitrogen, their antioxidative regulation and protection.
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-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Auditorium
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2
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Topics
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Oxidative stress, its types: adaptation, damage, repair and cell death.
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-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Auditorium
|
2
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Topics
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Local stress in organs and organ systems. Correction options.
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-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
|
Auditorium
|
2
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Topics
|
Local stress in organs and organ systems. Correction options.
|
-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
|
Auditorium
|
2
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Topics
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Oxidative stress and antioxidant defense: how does it work in practice?
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-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Auditorium
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2
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Topics
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Nutrition (dietary supplements) as a chronic stress regulator.
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-
Lecture
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Auditorium
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2
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Topics
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Quantitative and qualitative determination of active forms of oxygen and nitrogen and free radicals in the laboratory. Apparatus that can be used to measure markers of stress and indicators of antioxidant defense.
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-
Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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4
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Topics
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Total polyphenol; Quantification of DPPH and FRAP in biological materials.
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-
Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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4
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Topics
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Determination of Total Antioxidative Status in biological materials.
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-
Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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4
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Topics
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Determination of ABTS in biological materials.
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-
Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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4
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Topics
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Determination of selenium in blood plasma.
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-
Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
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Laboratory
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4
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Topics
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Determination of GSH or GSH/GSSG in biological materials.
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-
Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
|
On site
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Laboratory
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4
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Topics
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Determination of Hb in whole blood and erythrocyte lysate.
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-
Class/Seminar
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Modality
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Location
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Contact hours
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|---|---|---|
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On site
|
Laboratory
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4
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Topics
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Determination of MDA in biological materials.
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Total ECTS (Creditpoints):
6.00
Contact hours:
48 Academic Hours
Final Examination:
Exam (Written)
Bibliography
Required Reading
1.
Barry Halliwel and John M. C. Gutteridge. Free radicals in Biology and medicine. 5th ed. 2015., pp.936.
2.
Donald Armstrong, eds. Free Radical and Antioxidant Protocols., 1998, pp.455. (akceptējams izdevums)
3.
Donald Armstrong, eds. Free radicals in Diagnostic Medicine., 1994, pp.454. (akceptējams izdevums)
4.
G. Tirzītis, A. Šķesters. Skābekļa atvasinājumu un brīvo radikāļu bioķīmiskie aspekti bioloģijā. 2007, lpp.105. (akceptējams izdevums)
5.
Olle Selenius, Brian Alloway, Jose A. Centeno et al. Essentials of Medical Geology. Impacts of the Natural Environment on Public Health. ELSEVIER Acad. Press. 2005., pp. 812. (akceptējams izdevums)
6.
Redox Biochemistry. Eds. Ruma Banerjee; Donald Becker; Martin Dickman; Vadim Gladishev and Stephen Ragsdale, A. John Willey&sons, Inc., Publication
7.
Clinical Biochemistry. Metabolic and clinical aspects. 2nd edition, Eds by William J. Marshall; Stephen K. Bangert
Additional Reading
1.
Pub Med un citas datu bāzes.